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To mimic the niche and its cross-talk, scientists might use 3D-embedded assays with substances such as collagen or Matrigel, a gelatinous protein mixture secreted by mouse sarcoma cells.
The maximum growth inhibitory rate was 56.1% by 80 mg/kg/day on S180 mouse sarcoma cells; however, the maximum inhibitory potency on angiogenesis in C57BL/6 mouse subcutaneous Matrigel plug model was 50 mg/kg/day.
Finally, genetic silencing of ASNS in mouse sarcoma cells combined with depletion of plasma asparagine inhibited tumor growth in vivo.
In addition, oridonin enhances the efficacy of the cancer drug cisplatin in mouse sarcoma cells (Gao et al. 1993).
Further experiments showed that reducing the amount of asparagine in human and mouse sarcoma cells slowed down the growth of these cells.
SAIL-binding mouse mAbs were generated by standard hybridoma methodology after immunization with mouse sarcoma cells stably transfected with the human SAIL antigen.
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Cells from a LPB cell line, a methylcholanthrene-induced C57Bl/6 mouse sarcoma cell line [27], were cultured using standard procedures in MEM (Gibco BRL, Cer-gy-Pontoise, France) supplemented with 100 U.ml−1 penicillin, 100 mg.ml−1 streptomycin (Sarbach, France) and 8% foetal calf serum (Gibco).
Comparable hTNFα neutralising activity against a TNFα-sensitive mouse sarcoma cell-line (WEHI-164) has also been demonstrated.
Mouse sarcoma cell lines were established from 2 Kras;p16p19 null mouse RMS tumors (T14-R, SMP-01) and one Kras;p16p19 null mouse NMS tumor (Sca1-01).
For the Kd studies using flow cytometry, mouse sarcoma cell lines engineered to express full-length human SAIL were incubated with increasing concentrations of antibodies.
The S-180 mouse sarcoma cell line was obtained from the China Cell Culture Centre, grown and maintained in RPMI-1640 medium (Gibco) supplemented with 10% FBS.
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