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Cells from a LPB cell line, a methylcholanthrene-induced C57Bl/6 mouse sarcoma cell line [27], were cultured using standard procedures in MEM (Gibco BRL, Cer-gy-Pontoise, France) supplemented with 100 U.ml−1 penicillin, 100 mg.ml−1 streptomycin (Sarbach, France) and 8% foetal calf serum (Gibco).
For the Kd studies using flow cytometry, mouse sarcoma cell lines engineered to express full-length human SAIL were incubated with increasing concentrations of antibodies.
Mouse sarcoma cell lines were established from 2 Kras;p16p19 null mouse RMS tumors (T14-R, SMP-01) and one Kras;p16p19 null mouse NMS tumor (Sca1-01).
The S-180 mouse sarcoma cell line was obtained from the China Cell Culture Centre, grown and maintained in RPMI-1640 medium (Gibco) supplemented with 10% FBS.
Further work by other researchers in the 1980s supported this theory with studies of metastatic subclones from a mouse sarcoma cell line [ 26].
The mouse sarcoma cell line S-180 and macrophage cell line RAW264.7 were cultured in Eagle's Minimum Essential Medium and Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal bovine serum, respectively.
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Finally, genetic silencing of ASNS in mouse sarcoma cells combined with depletion of plasma asparagine inhibited tumor growth in vivo.
The maximum growth inhibitory rate was 56.1% by 80 mg/kg/day on S180 mouse sarcoma cells; however, the maximum inhibitory potency on angiogenesis in C57BL/6 mouse subcutaneous Matrigel plug model was 50 mg/kg/day.
Comparable hTNFα neutralising activity against a TNFα-sensitive mouse sarcoma cell-line (WEHI-164) has also been demonstrated.
In addition, oridonin enhances the efficacy of the cancer drug cisplatin in mouse sarcoma cells (Gao et al. 1993).
SAIL-binding mouse mAbs were generated by standard hybridoma methodology after immunization with mouse sarcoma cells stably transfected with the human SAIL antigen.
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