Sentence examples for mouse samples we from inspiring English sources

To compare expression levels in the human PDA tumor samples with mouse samples, we used the NCBI homologene database to extract mouse homolog genes for human genes.

In both human and mouse samples, we identified methylated cytosines in the promoter region of the heavy strand (PH) and within conserved sequence blocks (CSBI-III), which are highly conserved sequences located at the 5′-end of the D-loop and considered to be implicated in the processing of the RNA primer during the replication of the H-strand.

Our previous analysis of single mouse samples showed we obtained far more sequence depth than needed to identify a known mutation in a single lane of Illumina sequencing.

For long-range PCR on 12 mouse DNA samples, we designed a pair of primers on D-loop sequences on the mtDNA that did not show sequence similarity with nuclear DNA (forward primer: 5′- CCCAGCTACTACCATCATTCAAGT-3′; reverse primer: 5′- GAGAGATTTTATGGGTGTAATGCGG-3′) for the mtDNA.

Using single species RNA samples and mixed human-mouse RNA samples, we formulated and characterized two-step real-time RT-PCR assays to quantitate expression of the indicated transcripts and described analytical performance of the assays.

There were no archaeological mouse samples from which we could derive ancient DNA at L'Anse aux Meadows in Newfoundland and we can only speculate whether house mice arrived in the Viking period at all.

We were unable to reliably detect the mRON transcripts by RT PCR in mouse samples, but when we examined the splicing pattern of RON in human normal tissues and cell lines (Supplementary Figure S6) levels of exon 11 exclusion were overall lower in the tissues and more normal cell lines (IMR90 and HUVEC) but increased in other cell lines, in particular glioma cell lines U373 and T98G.

In addition, to obtain reagents that would be useful both in mouse and human samples, we had to design and synthesize two peptides that matched both the human and mouse sequences.

In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species.

By intra-cerebrally challenging APPSwe/PSEN1∆E9 transgenic mice with these samples, we demonstrated that the sole presence of Aβ aggregates, regardless of the presence of clinical signs, was sufficient to induce Aβ deposition.

For individual mouse samples (n = 6), we incubated 2×105 purified CD4+ T cells in triplicate with 1×105 naïve lymphocytes and 1 µg purified recombinant proteins (or peptide) in a total volume of 100 µl at 37°C.