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The distribution of PC1 scores for human samples showed no overlap with PC1 scores for mouse samples, suggesting that PC1 summarizes the variation in expression caused by species differences (Figure S1).
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When immobilized on a chemically modified glass slide or a TentaGel bead, 1 captured far more IgG antibody from some NOD mouse serum samples than most Swiss mouse (control) samples, suggesting that the antibodies it binds are indeed linked to T1DM.
FOXG1 expression above 1 TPM was found in 23% (231) and 30% (140) of human and mouse samples respectively, suggesting that the expression of this gene was limited to selected tissues (Additional file 1: Table S1).
Glycogen isolated from db/db mice has little variation between samples, suggesting that large α particles are unable to form.
Bands of corresponding size are found in the normal (C57) mouse skeletal muscle sample but not the MSTN KO samples, suggesting that they are specific myostatin bands.
The presence of elevated DR6 mRNA and protein levels in spinal cord of SOD1G93A mice and human ALS post-mortem samples suggests that increased DR6 levels may contribute to ALS pathology.
Hence, the consistent distinction of Heligoland mice across the three time-samples suggests that not only the size, but also the shape evolution has occurred early during the colonization phase and is not further ongoing.
The results suggest that proteomic experiments using more heterogeneous mouse samples would not require much larger sample sizes than those using narrowly standardized samples.
This suggests that the direct comparison of the wild-type and transgenic mouse samples on the same array drives the better performance, which is accordance with previous observations [ 13, 33].
The comparison between corresponding promoters in human and mouse samples, including the novel promoter p1@FOXG1 in human and pA@Foxg1 in mouse, revealed remarkably similar shapes, suggesting evolutionary conservation in their regulation.
This clearly suggests that if conclusions are to be drawn from mouse models of breast cancer, that the mouse samples should be compared and clustered with a variety of human tumors.
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