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Since the putative serum SEPs might show very low abundance and high dynamics, 11 WT mouse samples and four ob/ob mouse samples were chosen for the following MS detection, respectively (Fig. 3B, C).
DSS treated mouse samples indicated highly inflamed colon tissue with extensive mononuclear cells infiltrations in comparison to the tissue obtained from unexposed mice.
We investigated the effects of sample collection methods and storage conditions on bioactive IGF measurement using a modified kinase receptor activation (KIRA) assay in human and mouse samples.
The results suggest that proteomic experiments using more heterogeneous mouse samples would not require much larger sample sizes than those using narrowly standardized samples.
Appropriate results were previously obtained for these control mouse samples using Western blot and ECLISA.
Mouse samples were normalized towards β-actin and human samples towards GAPDH.
The immunoblot procedures for human and transgenic mouse samples were performed as previously described [19].
Apart from ES cells, mouse samples were isolated from 12-weeks old C57BL/6 animals.
The expression levels of 21,377 unique genes in a set of 2,543 diverse mouse samples were used.
Western blot analysis was performed on brain, shaven skin, lung, heart and liver prepared from B6D2F1 mouse samples.
GAPDH for human samples and rer1 for mouse samples have been additionally tested, in several cases, to confirm the results.
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