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Finally, a statistically significant difference is observed only for the kidneys between experimental and simulated mouse sample results.
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Consistently, SEP3 antibody detected stronger signal in WT mouse serum than that in ob/ob mouse serum, in agreement with the result that SEP3 was only detected in WT mouse samples in MS results.
Results indicated that phenotypic variability in the mouse sample population was the largest contributor to the standard error of the analyses.
Similarly, SEP54 antibody detected stronger signal in ob/ob mouse serum samples than that in WT mouse sample.
Since Abeta42 level is still low at this age of 3xTg-AD mice, many samples resulted in undetectable level: 2 out of 7 vehicle male, 6 out of 9 S14G-HN-treated male, none of vehicle female, and 5 out of 7 S14G-HN-treated female mice showed undetectable level of Abeta42.
Therefore, the reduced labelling for laminin in the Col4a1+/Svc mouse samples was most likely the result of reduced levels of laminin protein in the tendon.
Appropriate results were previously obtained for these control mouse samples using Western blot and ECLISA.
The results suggest that proteomic experiments using more heterogeneous mouse samples would not require much larger sample sizes than those using narrowly standardized samples.
GAPDH for human samples and rer1 for mouse samples have been additionally tested, in several cases, to confirm the results.
A Venn diagram (Figure 3B) summarizes the result we obtained by using the automated criteria to analyze 3 separate mouse samples.
As a result, for each cell type, we identified species-corrected DEGs upregulated in rat samples (in vitro; Fig. 6B) and in mouse samples (in vivo; Fig. 6D).
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