Mouse samples after 2 immunizations were tested for neutralization against these 2 RV strains, namely Wa (SGII, G1P1A[8]) homologous to the rVP6 used for immunization, and RRV (SGI, G3P5B[3]) (Fig. 2).
At least 4 representative mouse samples are shown.
B.W. performed protein expression studies in mouse samples.
M.P.C., J.R.P. and Y.L. prepared mouse samples for proteomics analysis.
Southern hybridization and PCR-based analyses of DNA from tissues of the resulting mice (sampled ∼1 month after TM treatment) confirmed the TM-induced, Cre-mediated excision of the Ate1flox allele in Ate1flox/− CaggCreER mice.
YPD was used as a selective medium for C. albicans because there was a possibility that some indigenous microorganisms in mice may contaminate the swabbed sample after disinfection by Chlorhexidine.
In studies using transgenic mice, Ad5 (10/0.1 mL per mouse) was injected intravenously into wild-type (WT) C57BL/6, GATA1 CAR, or GATA1 CR1 mice and blood sampled after 10, 30, 360, and 1440 minutes.
After washing with PBS, diluted mouse serum samples collected after each immunization were incubated at 37 °C for 1 h, followed by washing three times, 100 μL of horseradish peroxidase (HRP -conjugated anti-mouse antibody at different dilutions (1:100, 1:1000, and 1:10,000) were added to wells.
The titers of immunoglobulin G antibody specific to H7N9-M2e and H5N1-M2e were gradually increased in mouse serum samples collected after each vaccination, reaching very high levels (>1 10) 10 days after the last booster vaccination.
"We gave them sample after sample.
