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The authors thank M. Hattori, Y. Obayashi and K. Nakamura for their contribution to the TPR50 mouse sample preparation and analysis.
Instead of correlating tested samples to a single relative standard curve, serial dilution curves were constructed for every mouse sample.
Results indicated that phenotypic variability in the mouse sample population was the largest contributor to the standard error of the analyses.
Similarly, SEP54 antibody detected stronger signal in ob/ob mouse serum samples than that in WT mouse sample.
As demonstrated in Figure 5B, the propeptide antibody did not detect any bands in the mouse sample, despite equal protein loading (actin blot).
This is above the expected threshold observed in our house mouse sample for the occurrence of a prestyle, and indeed this species is characterized by an extreme elongation of the forepart of the UM1 and the occurrence of a prestyle.
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At least 4 representative mouse samples are shown.
B.W. performed protein expression studies in mouse samples.
M.P.C., J.R.P. and Y.L. prepared mouse samples for proteomics analysis.
Samples were then processed in the same manner as the mouse samples above.
The data for mouse samples were deposited in GEO database (GSE 92381).
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