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The results indicate an increased level of Gab1 phosphorylation in PTP1B knockout mouse retinas compared to wild type retinas.
The results indicate a reduced binding of Grb14 with the vSrc-SH2 domain in Ins2Akita mouse retinas compared to controls, suggesting that Grb14 phosphorylation is reduced in type 1 diabetic mouse retinas.
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We defined a bright green area as a YO-PRO-1 positive area (Figures 3B and 3D), and quantified YO-PRO-1 positive areas on rat and mouse retinas, comparing with those on acutely isolated retinas.
We identified 375 genes that showed differential expression in rods from 5 and 12 month old mouse retina compared to that of 1.5 month old retina.
Additionally, at least two, TRIM27 and TRIM23, demonstrate altered expression in Ataxin-7266Q/+ Ataxin-7266Q/+omoused with wild-type litteretina.
Previous studies have shown that Id1 and Id3 are important for retinal development: double-knockout mice display smaller retinas compared with WT at E13.5 (Du and Yip, 2011), and overexpression of Id1 and Id3 in the retina starting at P0 increases the number of cells that resemble retinal progenitors or Müller glia in the mature retina (after P14) (Mizeracka et al., 2013).
This was in agreement with expression pattern in normal mouse retina and in Nrl-/ retinas compared to wild type [ 53].
Total cholesterol levels were increased in cholesterol fed rabbit retinas compared to control retina.
In conclusion, degeneration of the RPE with subsequent photoreceptor degeneration by NaIO3 lead to longer mean fluorescence lifetimes of the retina compared to control mice, whereas during specific degeneration of the photoreceptor layer induced by MNU shorter lifetimes were measured.
Hence, in a recent study we showed that data variation introduced by biological replicates of the mouse retina is small compared to the variation caused by using different technologies [ 4].
The total number of calretinin-positive cells was significantly higher in the postischemic P2Y1R-KO retina compared with values found in Wt mice in both layers.
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