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The challenge assay was performed using the mouse respiratory model of B. pertussis infection as described previously [ 30].
Although it has been well established that Fim2 purified from B. pertussis confers protection against infection in the mouse respiratory model [ 20], mice intraperitoneal immunized with the recombinant protein (rFim2) are not protected against challenge with B. pertussis [ 42].
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We have recently established an in vivo model of Bordetella biofilms in the mouse respiratory tract [14], [15].
Fig. 2 The respiratory model.
Therefore, we made an experimental respiratory model to evaluate the respiratory physiological effect of HFNC.
Our data suggest that also in other AD mouse models respiratory function analysis needs to be addressed.
The major result provided by this study is the demonstration that DMSXL mice, a transgenic mouse model of DM1, have respiratory impairments characterized by a significant decrease in RR, TV, MV and SpO2 compared with control mice.
We tested the utility of our proposed methodology by using sequentially acquired CT and PET images of rabbit, ferret, and mouse models with respiratory infections of Mycobacterium tuberculosis (TB), H1N1 flu virus, and an aerosolized respiratory pathogen (necrotic TB) for a total of 92, 44, and 24 scans for the respective studies with half of the scans from CT and the other half from PET.
We also analyzed the two different MD mouse models for respiratory function.
Using a mouse model of respiratory tract infection, we previously found that PT is required early during the bacterial infection [24].
A mouse model of respiratory tract infection by this pathogen has been widely used to study the roles of various virulence factors, the associated pathology, and the immune responses elicited by the infection.
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