To further understand the mechanisms underlying MC-associated normalization of placentation, we next focused our attention on galectin-1 (Gal-1) that has emerged as a regulator of pregnancy and is abundant in human and mouse reproductive tracts.
Our results demonstrate that male and female mouse reproductive tissues stored in RNA later solution were stable and gave high quality RNA and protein after 10 months of storage at −80°C.
The second evaluation was performed at 12 weeks of age (three months), which is a post-pubertal time point when the peak bone mass has been mostly achieved, bone mass acquisition has slowed, and the mouse reproductive system is fully developed.
We are identifying estrogen-responsive genes in mouse reproductive tracts using cDNA microarrays and trying to establish microarray systems in the American alligator, roach, medaka, and water fleas (Daphnia magna).
We have begun to identify estrogen-responsive genes in the mouse reproductive tract using cDNA microarrays and to establish microarray systems in the American alligator, roach, medaka, and water fleas (Daphnia magna).
We compared transcript responses and mechanisms of response using mouse reproductive tracts after treatment with E2, estriol (E3), bisphenol A (BPA), and 2,2-bis p-hydroxyphenyl -1,1,1-trichloroethane (HPTE).
Rousset et al. first reported that UCP2 is expressed in the female mouse reproductive tract, which was detected in ovary, oviduct, and uterus [ 19].
We are grateful to C. Hodges for helpful advice and assistance with the analysis of mouse reproductive organs.
The proportion that went on to create healthy offspring was relatively low — five pups were born from 127 implanted embryos (3.9%) in the case of stem cells compared with 13 pups out of 75 embryos (17.3%) in one of the controls, which started with normal mouse reproductive cells.
To investigate B4galnt2 gene regulation in the female mouse reproductive system (B4galnt2 encodes an enzyme, β1,4-N-acetylgalactosylaminyltransferase II, that catalyzes the addition of GalNAc to glycoproteins via a β1,4 linkage).
