Exact(3)
The standards were serial dilutions of mouse reference serum (Bethyl Laboratories, Montgomery, TX, USA).
The final concentration in each tube was 19, 38, and 76 mg/mL albumin in PBS, and 42.5 mg/mL albumin in mouse reference serum (Bethyl Laboratories , Inc. with 29.8 ng/mL PbTx-3/[H]PbTx-3 (32,000 cpm).
After blocking of unoccupied sites on the plastic with bovine serum albumin, the test samples and standard mouse IgA (mouse reference serum; RS10-101, Bethyl Laboratories, Inc). were added.
Similar(5)
Concentrations of Ig isotypes (μg/ml) were calculated by the interpolation of calibration curves generated by using a mouse Ig reference serum (ICN Biomedicals, Aurora, OH).
For HSP70-specific antibody production a reference serum of HSP70 immunized mice was used.
The processing of data was performed without taking into account reference serum values of the drugs.
The OD intensity reduction caused by serum antibodies blocking mAb binding was calculated for each sample dilution by using the formula: % inhibition = [ negative reference serum OD−test serum OD)/(negative reference serum OD−positive reference serum OD)]×100%.
Equine reference serum was used as standard.
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