Exact(1)
For the Fu & Li's tests, we used the mouse reference mtDNA sequence (NC_005089) as outgroup.
Similar(7)
Sequencing reads from human samples were mapped to the human mtDNA reference NC_012920.1 using BWA (v.0.5.6) [ 29] and Samtools (v.0.1.18) [ 28], while sequencing reads from mice were mapped using the same software to mtDNA reference sequence in mouse reference genome build GRCm38.
Mutations were scored relative to the revised Cambridge reference mtDNA sequence [32].
Novelty is almost always operationally defined by searching for mtDNA in the main reference mtDNA database in the field, namely, MITOMAP (http://www.mitomap.org/cgi-bin/mitomap/search.pl) [20].
Of the 13 protein-coding genes in mtDNA, we obtained their strands from human reference mtDNA sequence (rCRS).
Approximately 66% of the reads were mapped to the bovine reference mtDNA sequence (BRS) [ 31].
We use the reference mtDNA sequence (GenBank: NC_001807.4) for the purpose.
This position is A in a non-coding region of the reference mtDNA sequence while it is G in a deposited mtDNA sequence (AF346989).
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