Exact(3)
Using these parameters, a uniform RSPD and a non-strand-specific model, we simulated 30 million reads of length 25 from the mouse reference gene set with our generative model.
Comparison of the mouse alpha-defensins in the three main mouse reference gene sets Ensembl, Mouse Genome Informatics (MGI), and NCBI RefSeq reveals significant inconsistencies in annotation and nomenclature.
In addition to the human pre-miRNA target genes predicted from human ESTs, we retrieved the sequences of 3' UTRs of mouse and rat genes from the mouse reference gene transcript sequences and rat reference gene transcript sequences (mouse.rna.fna, rat.rna.fna; RefSeq release 3)[ 22].
Similar(5)
We defined a pair of adjacent genes in the mouse reference genome as any two genes for which intergenic distance was below 1 MB (as 92% of the imprinted genes in our data are within 1 MB of another imprinted gene).
Using RNA-Seq, an average of 22 million short sequencing reads were generated per sample for 21 samples (10 B6 and 11 D2), and these reads were aligned to the mouse reference genome, allowing 16,183 Ensembl genes to be queried in striatum for both strains.
Designing unique primers for individual knockout of alpha-defensin genes is impossible and the exact complement of the alpha-defensin genes within the mouse reference genome (C57/BL6 strain) is still unknown.
We used BLAT to determine the genomic co-ordinates of each gene exon in the NCBIM37 mouse reference genome.
Reads were mapped to the mouse reference genome GRCm38 (release 74) representing 39,179 Ensembl annotated genes.
Related(1)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com