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BAM files plus the mouse reference genome were then loaded into the Integrative Genomics Viewer (Version 2.3.34) [ 34, 57] and read mapping along all chromosomes was inspected visually.
We used BLAT to determine the genomic co-ordinates of each gene exon in the NCBIM37 mouse reference genome.
The mouse reference genome (mm10) was obtained from the iGenomes repository (https://support.illumina.com/sequencing/sequencing_software/igenome.html.html
Raw readseq reads were aligned onto mouse reference genome mm10 using HISAT.
Tophat (v 2.0.10) was used to map sequencing reads to the mouse reference genome (mm9 RefSeq).
Reads were mapped on mm10 mouse reference genome and quantified using CLC Genomics Workbench version 9.5.1 and 10.1.1 (QIAGEN).
Following alignment to the mouse reference genome GRCm38 with BWA59, variants were called with GATK UnifiedGenotyper60, Varscan260, and Platypus61.
High quality raw sequence reads were processed and aligned to mouse reference genome mm10 using HISAT and output collected as SAM files.
Single cell or nuclear reads were aligned to the mouse reference genome (GRCm38) using STAR (2.3.0) and assembled and quantified by HTSeq (v0.6.1) using Gencode vM8 annotations.
The libraries were sequenced using Illumina MiSeq and the reads were mapped to the mouse reference genome (mm10) by the Bowtie 2 software (version 2.2.6 43.
All the GTFs previously obtained for each sample were grouped and provided as an input for stringtie "merge" mode along with mouse reference genome (mm10-Mus_musculus.GRCm38.84.gtf).
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