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For oxidative stress experiments, each mouse received a single dose of 20 mg/kg PQ (Sigma) (i.p). 7 days after Tx treatment.
The mouse received a pellet reward if it was poking in the later hopper after the light signal and before the time limit (30 seconds).
N-acetylcysteine (Sigma) was dissolved in phosphate-buffered saline (PBS) and each mouse received a single dose i.p. of 150 mg/kg, with 1 mg/ml in drinking water (ad libitum) for 4 months.
Two mice were immunized intramuscularly with six doses of 100 μg of plasmid at 2-week intervals; one mouse received a booster of live virus prior to the last plasmid injection.
Thus, each mouse received a total activity of 15 MBq (0.6 μg peptide, n = 5/group), which was chosen to avoid complete tumor remission.
Activating hippocampal neurons associated with a familiar cage and the amygdala neurons that fired when the mouse received a shock in the foot tricked the mice into later behaving as though they had been shocked in the cage, even though they had never been injured there.
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Sham mice received a noncoding plasmid.
32 male balb/c mice received a dorsal skinfold chamber.
Another eight mice received a sham treatment (SHAM group).
A third of the mice received a high dose of NAC in their drinking water.
Perioperatively mice received a subcutaneous injection of Rimadyl (Pfizer, 4.4 mg kg−1 in 0.1 ml of water for injections).
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