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Experiments in hippocampal slices from mouse pups provide useful information about cerebral edema and epilepsies in juveniles, but data may not be applicable to adults.
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Astrocytes isolated from postnatal mice pups were provided by Dr. Davide Trotti (Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA) following established techniques [ 21].
Primers used for real-time PCR analysis are provided Table 1.> Mouse pups of the desired genotype were subjected to a single intraperitoneal injection of either NGF (2 μg reconstituted in PBS with 1% BSA) or equal volume of 1% BSA in PBS at both P0 and P1.
Changes in syllable sequencing that occur during development may provide additional cues for adult animals to differentiate the calls of mouse pups from those of adults.
The sound is similar to one emitted by mouse pups.
Mouse pups (day 7 postnatal) were subjected to sevoflurane inhalation.
Primary mouse neurons were obtained from C57 mouse pups (sacrificed postnatal day 2–3).
Differential responses in the lungs of newborn mouse pups exposed to 85% or >95% oxygen.
Mouse pups were tested for their somatic development and reflexes at P2, P5 and P8.
However, there are no commercially available anesthetic or stereotaxic devices for mouse pups.
These were then successfully fertilised in the lab by IVF and the resulting mouse pups were healthy and fertile.
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