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As in the mouse protocol described above, the rats were randomly allocated into vehicle control or Cangrelor treatment groups.
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We next isolated the primary tumor cells from the PyVT mice, and examined their tumor development in athymic nude mice following the protocol described previously [39].
To determine the role of B cells in chronic allergic lung disease, we subjected B cell deficient (µMT) and wild-type mice to the protocol described in Fig. 1A.
All mice underwent the protocol described in Fig. 1.
The primary myoblasts were isolated from the leg muscles of adult C57BL/10 (an inbred substrain of C57BL/6) or C57BL/Ka-β-actin-EGFP mice using the protocol described by Rando and Blau [ 29].
cDNAs were synthesized from total RNA of both infected and uninfected mouse liver using the protocol described above.
The blood stage forms of both parasites were stored in liquid nitrogen after in vivo passages in C57BL/6 mice according to the protocol described elsewhere [31].
The blood stage forms of both parasites were stored in liquid nitrogen after in vivo passages in C57BL/6 mice according to a protocol described elsewhere [25].
We subjected wild-type mice to the AOM/DSS protocol described in Figure 2A.
RD was created in the eyes of 6- to 8-week-old mice, according to the protocol described previously with slight modifications.
Keratinocytes and dermal fibroblasts were cultured from wild-type newborn mice according to the protocol described previously (Rishi et al. [ 8]).
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