IPV-specific fecal IgA levels were determined by comparison with a mouse IgA protein standard (Southern Biotechnologies, cat #1040-08) included as a standard on each ELISA plate.
The rabbit polyclonal antibody to dysbindin-1 was generated by injecting rabbits subcutaneously with 1.8 mg of purified GST-dysbindin-1 (mouse) protein from E. coli using the standard immunization protocol.
We would like to note that these experiments were carefully designed and included purified recombinant protein standards spiked into respective mouse knockout retinas.
Mouse recombinant IGF-1, IGFBP-1, and IGFBP-3 protein standards, monoclonal antibodies and biotinylated polyclonal antibodies were purchased from R&D systems (Minneapolis, MN).
The concentrations of the cytokines and C5a were calculated from a standard curve of the respective recombinant mouse protein, by using Gen5 Data Analysis Software (BioTek Instruments, Bad Friedrichshall, Germany).
The mouse kinome: discovery and comparative genomics of all mouse protein kinases.
Though most of the transcripts code for mouse proteins, he found as many as 4,280 he could not match to any known mouse protein, suggesting that a large part of the genome consists of nonprotein coding genes.
Western blots were calibrated with protein standards.
After injections of mice with the purified protein, standard procedures were followed to obtain the antibodies.
Briefly, purified rat anti-mouse IgG1 mAb (A85-3; Phandingen) and HRP-conjugated rat anti-mouse IgG1 (Zymed, San Francisco, California, USA) were used for plate coating and secondary Abs, respectively, with mouse IgG1 S1-68.1 S1-68.1ingen) used as the PharMingenandard.
