Sentence examples for mouse protein sequence from inspiring English sources
For each homolog pair, we required a BLAST score of greater than 200, and at least 80% alignment of the human or mouse protein sequence with the rhesus sequence (Table S2).
The genomic location of the best match to the mouse protein sequence from the second genome was retrieved.
When this information did not exist, we used the mouse protein sequence of the identified gene for a translated BLAST (tblastn), or the nucleotide sequence of the cDNA fragment or the GenBank accession number of the corresponding gene for a blastn on the human genome.
By examining the number of single-copy orthologs identified from comparing translated coding sequences (CDS) of the guppy against other teleost, human, and mouse protein sequence databases, we identified a greater number of orthologs in the genome-guided assembly than in the genome-independent assembly.
Any mouse genes that did not have an ortholog in another genome identified in BioMart were then subjected to a BLAT search http://genome.ucsc.edu/cgi-bin/hgBlat command=start on the other genome using the mouse protein sequence retrieved with the BioMart sequences filter.ucsc.edu/cgi-bin/hgBlat command=start
For trans-alignment, human and mouse protein sequences from the UniProtKB database (http://www.uniprot.org/downloads) were aligned against the sequenced genome using BLAT [47] and filtered by 95% coverage cutoff.
On a genome wide scale, mouse protein sequences share 78.5% sequence identity with human counterparts [ 1].
In the case of the remaining 5 AQP4 transcripts, the chicken and mouse protein sequences were clustered with human transcript ENST00000339532.
Mouse protein sequences were compared to mouse cDNA sequences with the aid of computational translation using Vector NTI software (Analyses module, Translation).
The mouse protein sequences used to validate the human databases were also obtained from NCBI IgBLAST and processed in the same way as the human sequences.
A further 24273 human and 19258 mouse protein sequences were retrieved through the web interface [ 92] from the August 2006 version of the Mammalian Gene Collection.
