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Elucidation of the evolutionary constraints that have shaped fixation of transposed elements within human and mouse protein coding genes and subsequent exonization is important for understanding of how the exonization process has affected transcriptome and proteome complexities.
Sequences of TE exonizations within human and mouse protein coding genes were selected.
These SNPs involved 4862 human and eight mouse protein coding genes (Supplementary Table S5).
The Conserved CDS database (CCDS) [ 2] contains human and mouse protein coding regions that are consistently annotated in NCBI and Ensembl genome browsers and provides a gold standard for coding region locations.
NGS sequencing and data processing: exome capture from CT26 and BALB/cJ mice were sequenced in triplicate using the Agilent Sure-Select solution-based mouse protein coding exome capture assay.
There are many benefits of sequencing a genome, but for relatively large genomes such as human and mouse, protein coding regions account for under 5%, thus most of the sequencing effort would go to sequencing either regulatory regions or repetitive elements [ 2].
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For the analysis, we selected eight different mouse protein-coding genes covering different cellular functions.
We analyzed alternative transcripts from the human and mouse protein-coding genes deposited in two major databases: RefSeq and UCSC.
Then, we examined the distribution of sense and antisense mapped reads around the TSS of each mouse protein-coding gene.
The IKMC is currently mutating all mouse protein-coding genes in embryonic stem (ES) cells using gene targeting and gene trapping approaches.
We studied the relation between AARs and the Gene Ontology terms (GO), for Molecular Function, Biological Process and Cell Component, of all human and mouse protein-coding genes.
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