Mouse prostate tissues were fixed in 10% v/v formaldehyde in PBS, embedded in paraffin, and cut into 5 μm sections.
Slides containing sections of formalin-fixed, paraffin-embedded (FFPE) mouse prostate tissues were deparaffinized in xylene and rehydrated through a series of graded ethanol.
Mouse prostate lobes and tissue recombinants were dissected and fixed in 10% phosphate-buffered formalin overnight, transferred to 50% ethanol, then embedded in paraffin.
Nkx Pten fl/+ Spry2 +/− mice were generated and the prostate tissue was harvested from mice around 10 12 months old.
Moreover, IL-1 β activates NF- κB in prostate tissue in mouse models [ 48, 49].
These conclusions are supported by our recent observations that Pim1 expression resulted in mild pathological alterations in normal adult mouse prostate epithelial cells using a tissue recombination system, but it greatly accelerated the progression of c-MYC-initiated tumors [ 44].
Genomic DNA was isolated from the palpable prostate tumors of 24 weeks old TRAMP mice (n = 12), apparently normal prostate tissue of 24 weeks old C57BL/6J mice (n = 10), TRAMP-C1 and C3 cells using the DNeasy tissue kit (Qiagen, Valencia, CA).
An increased expression level of CCN4 has been found in prostate cancer tissue in the early stages, sera of patients, and carcinoma tissues of the mouse prostate cancer model TRAMP, which spontaneously develops to prostate carcinomas.
