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In this study we sought to identify aging-associated alterations in the mouse prostate microenvironment that could influence pathology.
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Transgenic adenocarcinoma of the mouse prostate.
We used animals designed to develop this disease, Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mice, to investigate the potential role of AHR signaling in prostate cancer development.
Primary prostate colonies from DsRED transgenic mouse prostate cells were trypsinized and dissociated into single cells.
In summary, the studies of the murine prostate microenvironment reported herein demonstrate consistent molecular and structural changes that accompany advancing age.
Study of tumor development in mouse prostate cancer models can be instrumental in understanding human prostate cancer.
RNA from microdissected mouse prostate stroma and epithelium, and human prostate fibroblast (PSC27) were used as template for qRT-PCR.
A balanced IL-2 cytokine production in the prostate microenvironment may thus contribute to prostate cancer susceptibility.
We examined the effects of lycopene and fish oil supplements versus placebo on the normal prostate microenvironment, among men pursuing active surveillance for low-burden prostate cancer.
Moreover, overexpression of Skp2 in mouse prostate leads to prostate intraepithelial neoplasia (PIN) [50].
Mouse prostate lysates were sonicated prior to protein quantification.
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