During the course of our study, the authors become aware of the work of Van Bergen et al. [23] that cast uncertainty on whether the origin of the RGC-5 cell line is rat or mouse (although, elevated pressure is known to induce RGC apoptosis in both species [11], [24]).
Following injection of these mutants into mice, antibiotic selective pressure is relaxed, leading to an increased chance of TGR.
The goal was to assess effects of IOP and pilocarpine-induced ciliary muscle contraction on conventional outflow pathway tissues in living anesthetized mice.Intraocular pressure was controlled by intracameral cannulation of mouse eyes while imaging using spectral-domain optical coherence tomography (SD-OCT).
However, the increase in arterial pressure in reproductively senescent female mice is apparent at 18 months of age, at which time the sexual dimorphism in arterial pressure is lost in mice (arterial pressure measured via radiotelemetry, unpublished data).
Blood pressure is reduced substantially in the Agtr1a -/- Agtr1b -/- mice and following administration of an angiotensin converting enzyme inhibitor, their blood pressure increases paradoxically.
Mouse diastolic blood pressure was calculated by the software MRBP Monitor Version 1.59 (MRBP-MMC, IITC Life Science Inc, CA, USA).
In LF-fed mice, systolic blood pressure was significantly elevated (8 mmHg) in GRβ geo /+ mice compared with their GR+/+ littermates (Fig. 7 ).
Animal measurements Four weeks after infusion into Apoe−/− mice, systolic blood pressure was measured using indirect tail-cuff equipment (MK-2000; Muromachi Kikai, Tokyo, Japan) [ 14].
To rule out if the improvement of cardiac phenotype was due to fasudil-mediated vasodilation, mouse blood pressures were compared among sham, TAC, and TAC plus fasudil groups in WT and Rnd3+/− haploinsufficient mice.
In another experimental aging study on C57BL/6 female mice [ 26], the pressure was augmented in 16-month-old mice compared to 6-month-old group.
