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Specifically, we examined features corresponding to the 78 k mouse pool for signal when the 78 k human pool was hybridized.
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Extensor digitorum longus (EDL) muscles were dissected with their tendons from each control and tumor-bearing mouse, pooled and treated as described elsewhere [ 43].
Notably, little signal above the background defined by the Swiss mouse pooled sample was observed when serum samples from the Swiss8 and Swiss10 mice, which gave false positive results using immobilized 1, were incubated with the GAD65-GST-displaying beads.
One sample consisted of tear washings from both eyes of one mouse pooled (2 μL) in PBS + 0.1% bovine serum albumin (BSA) (8 μL) and stored at −80°C until the assay was performed.
One sample consisted of tear washings from both eyes of one mouse pooled (2 μl) in PBS+0.1% BSA (8 μl) and stored at -80°C until the assay was performed.
lpr/lpr mice pool.
Using high-throughput reduced representation bisulphite sequencing (RRBS; Meissner et al, 2008; Bock et al, 2010; Gu et al, 2011), we generated DNA methylation maps for pooled FACS-isolated, Sca1+Lin− fractions from Sca1-MafB mice and control mice, pooled B220+ cells from Sca1-MafB mice and control mice, and pooled Gr1+Mac1+ cells from control mice.
Serum samples were collected from mice, pooled and mixed, and kept on ice.
Lumbar L3-L6 doroot root ganglia (DRG) were dissected from CCI and control mice, pooled and total RNA extracted using the RNeasy mini kit (Qiagen, Germany).
We used a contingency table to test whether more mouse-scented plots (two mice and six mice pooled) were visited than control-scented plots after two days and also by the end of the sampling period.
Subcutaneous inguinal white adipose tissue was isolated from eight week old C57BL/6 mice, pooled, and minced in HEPES-buffered DMEM (Invitrogen) supplemented with 10 mg/ml fatty acid poor BSA (Sigma).
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