Sentence examples for mouse podocalyxin when from inspiring English sources
Additionally, we have not observed any adverse effect of antibodies targeting mouse podocalyxin when systemically administered to wild-type mice.
The cells were then harvested for analysis of expression of mouse podocalyxin RNA and protein.
Using shRNA, we silenced mouse podocalyxin surface protein expression by 6.3-fold in 4T1-luc mouse mammary tumor cells (BALB/c mouse-derived).
Polyclonal goat α-mouse podocalyxin (1∶50, R&D Systems) and monoclonal mouse α-pan cytokeratin antibodies (1∶50, Sigma) were used according to the manufacturer's instructions in combination with the appropriate secondary antibodies.
A goat anti-mouse podocalyxin antibody (2.5 μg/10 cells; R&D Systems) and an isotype against normal goat IgG (gray) were used.
Murine 4T1-luc cells were labeled with allophycocyanin-conjugated rat anti-mouse podocalyxin Ab (R&D Systems) and analyzed by flow cytometry.
MCF7pIRES control and MCF7Podxl lysates were resolved by SDS-PAGE and analyzed by Western blotting using a goat anti-mouse podocalyxin antibody (1 μg/ml; R&D Systems).
"That's what a mouse feels when it dies".
Release the left mouse button when satisfied.
P1A transgenic mice were sacrificed when moribund.
Podocalyxin-knockout mice shew that podocalyxin maintains the unique podocyte morphology because in these mice, there were fewer major processes, and lack foot processes and slit diaphragms [ 7].
