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Mouse perfusion, tissue processing and analysis were performed as previously described (Gomez-Nicola et al., 2011).
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However, mice lacking a functional eNOS enzyme, as present in eNOS-/ and double KO mice, perfusion in jejunal tissue did not decrease during LPS infusion.
For mouse perfusion, no second ligature is necessary (Fig. 57 d).
For mouse perfusion, the vena portae is cut immediately.
The following steps refer to the vena portae for rat perfusion and to the vena cava for mouse perfusion.
Mouse tissue was fixed by transcardial perfusion of 4% paraformaldehyde (PFA) in PBS.
Through our ex vivo artery perfusion method, we were able to simulate physiological flow and initiate distinct fluid shear stress mechanosensory responses, as well as induced acetylcholine responses in mouse aortic tissue.
Mouse liver perfusion is in principle similar to rat liver perfusion; however, the perfusion apparatus has to be miniaturized and the liver perfusion is preferably performed with the liver in situ due to the small animal size.
At 12 months of age, mice were injected with EB forty minutes before perfusion and tissue was collected following this in an identical manner to the cohorts described above.
Anaesthetized male mice at post-natal day 56 were fixed by perfusion with tissue fixative (GenoStaff, Tokyo, Japan) and the whole brain was embedded in paraffin.
To further correlate the impact of MARCKS phosphorylation on localization of Clca3 in ependyma, 2M and 2Y wild-type mice were intraventricularly injected with PMA followed by immediate perfusion and tissue analysis as described above.
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