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For mouse perfusion, no second ligature is necessary (Fig. 57 d).
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For mouse perfusion, the vena portae is cut immediately.
The following steps refer to the vena portae for rat perfusion and to the vena cava for mouse perfusion.
The cotyledon of perfusion no. 3 was leaking towards the end of the perfusion, therefore only data from the first 60 min of this perfusion have been used.
These are reduced perfusion, no perfusion response and increased perfusion also referred to as vascular normalisation (Mehta et al, 2011; Van der Veldt et al, 2012; Batchelor et al, 2013).
Mouse perfusion, tissue processing and analysis were performed as previously described (Gomez-Nicola et al., 2011).
FPL carried out the mouse perfusions, separation of worms and RNA extractions.
However, mice lacking a functional eNOS enzyme, as present in eNOS-/ and double KO mice, perfusion in jejunal tissue did not decrease during LPS infusion.
In addition, normal database of myocardial perfusion for mice is no longer required.
Mouse liver perfusion is in principle similar to rat liver perfusion; however, the perfusion apparatus has to be miniaturized and the liver perfusion is preferably performed with the liver in situ due to the small animal size.
The perfusion showed no clear correlation with LC (p = 0.09).
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