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To induce an acute cerebral infarction, a small region in the mouse parietal cortex containing multiple blood vessels was clotted by Rose Bengal (RB) induced photothrombosis (Fig. S1).
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50, 51 A limitation of our mouse experiments is that all spectral analyses were performed on EEG recordings obtained over the parietal cortex of resting mice.
The cell bodies of individual astrocytes were easily imaged with a confocal microscope through a thinned skull window in the parietal cortex of these mice (Fig. S1 and S2).
Neurotrauma on mice was induced using the controlled cortical impact model as in Samson et al. In brief, a burr hole was drilled over the left parietal cortex of anaesthetised mice and a single blunt impact (5 m/s velocity; 2 mm impact depth; 150 ms dwell time) was delivered by a controlled impactor device.
For primary isolation into transgenic mice, a 10% (w/v) homogenate of parietal cortex (UK-1) or frontal cortex (UK-2) was prepared in sterile saline.
Sham-operated mice received a circular craniotomy, and the parietal cortex was exposed.
Torin 1 administration normalized mitochondrial accumulation in the hippocampus of VGB-treated mice, but not in eye, liver, and parietal cortex of the same subjects.
Similar results were obtained with APPSwedish transgenic mice, revealing significant plaque reduction in the hippocampus and parietal cortex accompanied by alterations of APP cleavage products [ 113].
APPswe/PS1ΔE9 double transgenic mice display degeneration of 5-HT fibres, while APPSw,Ind transgenic mice overexpressing APP Swiss/Indian mutations show stable 5-HT fibre density in parietal cortex and the hippocampus.
Increased levels of APP mRNA and of the protein itself have been detected in the Ts65Dn mouse in the striatum by 6 8 months of age, and in the hippocampus and parietal cortex by 13 16 months of age.
Learning ability in the old mice also correlated with the ratios of mRNA expression for ϵ1 and ϵ2 and/or ζ1 subunits in the parietal cortex and CA1 region of the hippocampus.
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