Previously, we have demonstrated that ASCs of mouse origin successfully heal a critical sized mouse defect [30].
First we established that the line was of mouse origin, by using primers specific for mouse ß-globin and rat ß-actin against a genomic DNA template.
As no expression of human-specific CD31 was detected in the tumor sections (data not shown), we suspected that tumor angiogenesis was of mouse origin in this xenograft model.
We also examined the possible αSD detection of mouse origin, in sick M83 mice using a mouse specific antibody, D37A6.
To address this issue, we fused two cell types that are both of mouse origin but from different strains.
The M13 iPSCs, originated from SD outbred rat, and feeder cells of mouse origin remained in the prepared cell suspension, as allografts and xenografts respectively, may induce rejection in recipient SD rats.
In the current research, we have used a murine retrovirus-induced tumor cell line of C57BL/6 mouse origin, namely FBL-3 cells, as a model to study basic mechanisms of immunological control and escape during tumor formation.
Note, busulfan-depleted seminiferous tubules bear the risk of incomplete germ cell depletion resulting in infrequent mouse germ cells present; thus, we carefully discriminated positively stained germ cells that are of human or mouse origin (Fig. 5A) in all of our analysis and subsequent quantification efforts.
In the current research, we have used a highly immunogenic FV-induced tumor cell line of C57BL/6 mouse origin, namely FBL-3 cells, as a model to study the mechanisms of immunological control and escape during tumor formation.
We confirmed through RT-PCR that cell cultures were not contaminated with cells of mouse origin that might share identity in gene probe sequence [ 21].
