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To examine whether or not the injected agrobacteria are able to express in mouse organs, we used an intron-containing GFP (GFPi) reporter driven either by a cytomegalovirus (CMV) promoter or by a CaMV 35S promoter.
Since certain xenotransplantation experiments require an exact quantification of human cells in mouse organs, we developed in parallel a TaqMan-chemistry based real-time PCR approach amplifying a smaller fragment of the same genome localisation and using similar reaction and cycle conditions.
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Furthermore, from analysis of microarray database and immunohistochemical staining of GRP94 expression in mouse adult organs, we present predictions where GRP94 may play an important role in specific adult organ homeostasis and function.
In an investigation of the early changes induced in mouse organs exposed to a carcinogen, we determined whether DNA adduct formation, mRNA or miRNA expression can distinguish those mouse organs that are targets for BaP carcinogenesis (lung, spleen, forestomach) from three organs that are non-targets (liver, colon, glandular stomach).
When we compared the expression of miR-3099 in various adult mouse organs to the P150 whole brain, we found significant differences in the expression levels among the organs (P < 0.001).
Given the conservation of expression between mouse and human organs, we asked if this is also true for more distant vertebrates.
Before we applied the PPA to the human-mouse data we merged human and mouse organs into 27 HOGs.
In this study, we showed that several mouse organs express multiple prominin-1 splice variants on mRNA, indicating that these different variants probably have partially overlapping functions.
To study gene expression evolution between mouse and human we merged human and mouse organs into 27 HOGs.
Unfortunately, apart from the liver we did not harvest RNA from other mouse organs to co-investigate hepcidin-2 expression in our models.
Using the mapping available in the Bgee database we could map 36 human organs and 30 mouse organs to 27 HOGs.
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