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At 700nm, scattering coefficients for mouse organs are between 6.48mm−1 and 23.4mm−1 and the absorption coefficients between 0.0027mm−1 to 0.23mm−1 [ 10].
Instead, the results indicate that the profiles of BaP-induced miRNA expression changes in the six mouse organs are tissue specific.
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We further compared the levels of MIR168a in perfused and non-perfused organs after rice feeding and found no difference (Supplementary information, Figure S2D), which indicates that the elevation of plant miRNAs in mouse organs was not due to contamination by blood cells.
Although bioaccumulation of ACPNs has not been studied in particular, the distribution of HANs in mouse organs was studied via intravenous administration.
Mouse organs were examined histopathologically by veterinary pathologists (MR, HP, LM) using formalin-fixed, paraffin-embedded sections stained with hematoxylin and eosin (H&E).
RNA from mouse organs was prepared as previously described using a single step isolation protocol [51].
After perfusion, xenograft tumors and mouse organs were removed and homogenized.
Mouse organs were fixed in 4% paraformaldehyde overnight and embedded in paraffin.
To assess the overall ratios of ERM protein expression, various mouse organs were analyzed by western blot.
The infection of different mouse organs was monitored by demonstrating the presence of bacterial DNA by nested PCR (Table S1 and S2).
In order to investigate if the failure to grow in the mouse organs is due to the inability of the mutant to survive inside the macrophages, we examined the growth and survival of the mutant in resting and IFN-γ activated murine bone marrow derived macrophages which generate an oxidative burst by producing ROS and RNS [37].
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