Sentence examples for mouse oocytes of from inspiring English sources

We have shown that deletion of the Pten gene from mouse oocytes of primordial follicles using transgenic mice expressing Cre recombinase mediated by the growth differentiation factor 9 (Gdf-9) promoter, results in excessive activation of the entire pool of primordial follicles, which in turn leads to premature depletion of all primordial follicles [6].

The plasmid was digested with AscI and SspI, and the resultant 4.4-kb fragment was injected into fertilized mouse oocytes of C57BL/6 mice (Japan SLC, Hamamatsu, Japan).

Here, we isolated CCs from antral mouse oocytes of known developmental incompetence (NSN-CCs) or competence (SN-CCs) and compared their transcriptomes with the aim of identifying distinct marker transcripts.

The particular sensitivity of the maternal Mest allele to epimutations by low BPA concentrations may relate to influences of BPA on bidirectional signalling between the oocyte and its surrounding cumulus granulosa cells by gap junctional communication, as altered methylation of Mest was also detected in mouse oocytes of connexin 37 deficient transgenic mice [ 82].

To this regard, we isolated CCs from fully-grown antral mouse oocytes of known developmental incompetence (NSN-CCs) or competence (SN-CCs) and compared by microarrays their whole transcriptome and, by quantitative Real-Time PCR (qRT-PCR), the expression profile of CC-related genes (Has2, Ptx3, Tnfaip6 and Ptgs2).

To evaluate the effect of vitrification of mouse oocytes on the behavior of adult offspring.

Comparison of protein expression profiles of mouse oocytes to mouse embryonic stem cells (mESCs) showed high similarity between immature and mature oocytes on one hand, and between fertilized oocytes (zygotes) and mESCs on the other hand [ 13] which may be explained by the activation of the molecular programme in oocytes transiting into the embryo.

During mouse oocyte maturation, coincident with the cortical localization of the meiotic spindle was the formation of a microvillus- and cortical granule-free area and a thickening of the actin layer in this region of the egg cortex [10], and it was found that most of the mouse oocytes completed formation of the cortical granule-free domain at the metaphase I stage [63].

Samples of mouse oocytes at different stages of meiosis were collected after oocytes had been cultured for 0, 2, 5, 8, 9.5, and 12 h, corresponding to germinal vesicle (GV), germinal vesicle breakdown (GVBD), prometaphase I (Pro-MI), Metaphase I (MI), anaphase/telophase I (ATI), and metaphase II (MII) stages, respectively.

In mouse oocytes, the loss of Ago2 can result in decreased levels of siRNAs and increased levels of retrotransposons [ 97].

Most interestingly, when basonuclin was knocked-down in mouse oocytes, the number of Pol I transcription foci were reduced, and the incorporation of BrU by the remaining foci was not affected [6].