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However, pHi does not change significantly in mouse oocytes during meiosis or egg activation [60], [61], appearing to rule out such a role.
However, we found that DNMT3A2 is predominately expressed in mouse oocytes during the growth phase, suggesting DNMT3A2 is responsible for DNA methylation imprints in female germ lines as well as male.
JmjD3 is expressed in human, rhesus monkey and mouse oocytes during metaphase [ 194], and plays a role in oocyte preimplantation (e.g., in bovine fertilization), since knockdown of JmjD3 in oocytes inhibited H3K27me3 demethylation and impacted blastocyst [ 195], and later endoderm and mesoderm development.
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These results show that subcellular localization of Ae2 in mouse oocytes varies during meiotic progression, and that membrane localization of Ae2 is low in MII eggs relative to GV oocytes or fertilized eggs.
We believe that this study will help us to understand the diverse biological processes occurring in mouse oocytes and during early embryo development.
The distribution of PLCζ within the activating mouse oocyte changes during the first cell cycle, exhibiting PN localisation during zygotic interphase [ 66, 94- 97] thereby restricting access of the enzyme to its substrate PIP2, and thus resulting in termination of Ca2+ oscillations.
Lowther et al. reported that disruption of endoplasmic reticulum during vitrification of mouse oocytes and deficiencies in factors involved in endoplasmic reticulum reorganization during oocyte maturation could contribute to the low development potential of vitrified in vitro-matured oocytes [ 49].
However, we showed here that PAR6, one kind of PAR protein, was localized in the nuclei of mouse oocytes that formed primordial follicles during the perinatal period, suggesting a new role of PAR protein.
However, in mouse oocytes, I2PP2A localized to centromeres during both meiosis I and meiosis II [8].
Besides its high protein expression in mouse oocytes, little is known about Hsp90b1 during the transition from oocyte-to-embryo.
Mouse oocytes accumulate maternal mRNA and proteins during a growth phase of nearly two weeks that are similarly required during ovulation, fertilization, and early embryonic divisions.
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