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Mouse on Mouse (M.O.M).
Mouse on mouse kit (Vector) was used for staining with embryonic myosin heavy chain according to manufacturer instructions.
The sections were incubated for 1 h in 3.6% MOM blocking reagent (Mouse on Mouse immunodetection kit; Vector Laboratories).
Immunofluorescence staining involving mouse primary antibodies was performed using a Vector Mouse on Mouse Kit (Vector Laboratories, Inc., Burlington, ON, Canada).
After washing, the sections were incubated for 1 h in 3.6% MOM blocking reagent (Mouse on Mouse immunodetection kit; Vector Laboratories).
The tissue sections were washed again, incubated for 5 min in MOM diluents (Mouse on Mouse immunodetection kit; Vector Laboratories), and then incubated overnight at 4°C in MOM diluents containing the anti-CAMKIIα antibody.
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Detection was done with Vectastain Elite ABC kit or Vectastain Mouse-On-Mouse kit and the diaminobenzidine substrate (DAB Detection kit, all from Vector Laboratories).
Sections were blocked for 30 min with 10% goat serum or commercial blocking agent (from Vectastain Mouse-On-Mouse kit) in PBS-T.
A mouse-on-mouse immunodetection kit (Vector Laboratories, Burlingame, CA) was used.
Foxj1 was detected on paraffin sections with anti-Foxj1 antibody (1∶100; [42]) using the mouse-on-mouse (MOM) kit (Vector Laboratories), followed by hematoxylin counterstain.
Mouse IgG from the first detection was blocked using the mouse-on-mouse blocking kit (Vector Laboratories) before detection of BrdU.
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