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One mouse of each control pair was kept undisturbed and called "companion of health partner" (CHP).
One mouse of each C pair was called "companion of health partner" (CHP) remaining undisturbed; the other was injected with NaCl 0.9% (0.1 ml/10 g).
Each isoflurane-anesthetized mouse of each group received 50 μl of a 24 mg/ml RES solution (1.2 mg/mouse, ~60 mg/kg) through intranasal instillation as described elsewhere37.
Sagittal (top) and transverse (bottom) views of one mouse of each group obtained 45-60 min p.i.
At the end of the experiment, one representative mouse of each group was sacrificed by cervical dislocation and percentage of fat mass was determined by Dual-Energy X-ray Absorptiometry (DXA) (PIXIMUS™, LUNAR, Lambesc, France).
Since variability of the BoNT gene and protein sequence within botulinum neurotoxin subtypes A has been reported and these sequence variations impact on antibody binding and neutralisation [21], the capacity of mouse and chimeric antibodies to neutralise BoNT/A2 and /A3 was evaluated using a mouse neutralisation assay with 5 estimated MLD50 per mouse of each toxin subtype.
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Then, six mice of each group were imaged at doses of 7 and 13 Gy (total scan time <5 min).
P38α+/− and P38α−/− mice were challenged with PHZ to induce anemia, on day 4 of recovery, erythroblasts (CD71highTer119+) from three mice of each genotype were sorted and sent to Miltenyi Biotec Inc Auburnn, CA).
Bars are means+s.e.m. (*P<0.05, **P<0.01 and ***P<0.001 relative to untreated control mice of each genotype; +P<0.05, ++P<0.01 and +++P<0.001 relative to same treatment of the wild-type group; analysis of variance with Tukey's post hoc test).
Representative images of samples taken from five mice of each genotype.
At each time point, 1 to 4 TG and WT mice of each model were imaged (Table 1).
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