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DAPI staining reveals the origin of each nucleus (mouse nuclei show a characteristic pattern of large heterochromatin foci).
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His C mutant nuclei show mean intensity value of 4N nuclei, suggesting that mutant cells completed genome duplication.
Not all nuclei show the appearance of these structures.
10.7554/eLife.05116.004 Figure 2. AD cortical nuclei show increased DNA content variation (DCV) by flow cytometry.
Furthermore, some nuclei show slow basal-to-apical migration during S-phase, although most remain stationary.
Other thalamic nuclei show moderate or low dopamine innervation (Fig. 1).
(A2 – B2 ) Higher magnification confocal images of the cerebellar nuclei show transfected GABAergic and glycinergic neurons, respectively.
The percentage of nuclei showing fragments was calculated considering 1,000 nuclei.
Comparison of Hoechst stained nuclei showed bright anti-nucleolin staining localized to regions of marginalized chromatin.
The nuclei showed spread and dumbbell shapes (Fig. 4C).
Samples with >90% nuclei showing signals were considered informative.
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