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Melanocyte differentiation is usually studied on quail embryo skin and on mouse neural crest cell lines.
Osteoblasts from mouse neural crest derived frontal bone displayed a greater proliferative and osteogenic potential and endogenous enhanced activation of FGF signaling compared to osteoblasts from mesoderm derived parietal bone.
Given the unique qualities of neural crest cells and the clear delineation of the embryonic origins of the calvarial bones, we sought to determine whether mouse neural crest derived frontal bone differs in biology from mesoderm derived parietal bone.
Performing transplantations of mouse neural crest cells into the chick embryo, Mitsiadis et al. [ 15] showed that avian dental epithelium can still induce a nonavian developmental program in mouse neural crest-derived mesenchyme, resulting in tooth germ formation.
Interestingly, both Cx43 and N-cadherin appear to regulate mouse neural crest cell motility, perhaps by engaging p120ctn signaling [ 62].
For example, p120 catenin-related protein was co-localized with Cx43 and N-cadherin in mouse neural crest cells [ 96].
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Neuro2a is a mouse neural crest-derived cell line that has been widely used as an experimental model for neuronal differentiation study.
Neuro2A is a mouse neural crest-derived cell line that has been extensively used to study neuronal differentiation, and specification into different neuronal lineages is possible (Tremblay et al. 2010).
Overexpression of N-MYC in the mouse peripheral neural crest of the TH-MYCN mice gives rise to NB tumors that recapitulate many of the histological and pathological aspects of human NB [18] [20].
This response is consistent with the Zeb2-null mouse where neural crest cells, expressing Sox10, are induced but unable to migrate from the epithelium (Van de Putte et al., 2003).
NET expression in mouse embryonic neural crest cells is regulated by the autocrine growth factors, neurotrophin-3 (NT-3), fibroblast growth factor-2 (FGF-2) and transforming growth factor-β1 (TGF-β1; ref. [ 10]).
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