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MstnPP aggregates and protofibrils have a cytotoxic effect on mouse myoblasts when added to the culture medium.
Significantly, MstnPP aggregates and protofibrils have a negative effect on the viability of C2C12 mouse myoblasts when added to the culture medium.
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In another but more controversial example, the canonical TFIID complex is replaced by a smaller 150 to 200 kDa complex consisting of TBP2/TRF3 and TBP-associated factor 3 (TAF3) when mouse myoblasts differentiate into myotubes in culture [ 89].
Upper panel: Time-sequence images showing the process of segregation in mixed co-cultures of C2C12 mouse myoblasts (red) and HaCaT human keratocytes (green).
We identified and quantified (i) metabolic differences between genetically engineered human cell lines, (ii) alterations in cellular metabolism induced by differentiation of mouse myoblasts into myotubes, and (iii) metabolic changes caused by activation of neurotransmitter receptors in mouse myoblasts.
We further seek to identify the expression kinetics of various periostin isoforms during the differentiation of rat and mouse myoblasts.
In our latest experiment we targeted the last exons of the nebulin transcript produced by C2C12 mouse myoblasts.
Interestingly, reduction of vinculin expression was also observed in CAG200-expressing mouse myoblasts (unpublished data).
C2C12 mouse myoblasts (ATCC) were cultured in DMEM/10% FBS (GIBCO) until they reached 70% confluency.
Lamin A negatively affects epigenetic regulation of the myogenin gene expression in mouse myoblasts [36].
Second, immobilized GST-PARVB could pull down endogenous CAPN3 from IM2 mouse myoblasts, and vice versa (Figure S2).
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