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Evaluation of COMT activity in rat and mice liver homogenates revealed that both had similar methylation efficiencies (Kcat values, respectively 7.3 and 6.4 min−1), but rat had twice active enzyme units as the mouse (molar equivalency respectively 150 and 83).
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AlQ doses were 1 1000 μg per plate while 8OHQ doses were 0.947 947 μg per plate to maintain molar equivalency.
The mouse molars shown in Figure 7 are postnatal day 3 (P3).
We develop such an approach on mammalian dentition, and more specifically on the house mouse molars.
The supernumeraries usually have a reduced cusp pattern and do not resemble normal mouse molars.
Control mice received either saline or non-cell penetrating cN50 peptide at molar equivalent (20 µg/g).
C57BL/6 mouse first molar enamel organs were extracted for quantitative real-time PCR analysis.
The bacterial suspension was applied to the gingival margin of mouse maxillary molars as detailed below.
Young (3 4-month-old) mice were given a single i.v. injection of a molar-equivalent dose of biotinylated GDF11 (8 mg/kg), transferrin (25 mg/kg) (Sigma Aldrich) or albumin (22.5 mg/kg) (Sigma Aldrich).
Old (19 21-month-old 19 21-month-old 19 21-month-oldinjection of a micer-equivalent dose of biotinylated GDF11 (1 mg/kg or 8 mg/kg), transferrin (3 mg/kg) or albumin (3 mg/kg).
The mouse mandibular first molar tooth germ can inhibit second molar development and an inhibitory cascade model in which initiation of posterior molars depends upon a balance between intermolar inhibition and mesenchymal activation to determine sequential molar initiation has been proposed.
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