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Yet, the NOD.scid mouse model still did not support high rates or long-lasting reconstitution with human immune cells, nor did it allow development of functional human T and B cells [11].
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Nevertheless, specific muscle studies in this knockout mouse model are still required to exclude a muscle associated phenotype.
The reason for metastasis into regional lymphnodes in this mouse model is still unclear.
Although progress in generating GD animal models has accelerated significantly over the past decade or so, the 'perfect' GD mouse model is still lacking.
While tumor dynamics, vasculature and heterogeneity affect drug distribution, this non-orthotopic mouse model is still limited in fully representing the conditions in human patients.
Even though the mouse model is still considered more relevant for humans than ZF, it is much more difficult and expensive to manipulate mouse embryos, which need a uterine environment to develop properly and are much more expensive to produce in large numbers compared to ZF embryos [ 65, 72].
Nevertheless, mouse models are still underutilized for the genetic dissection of renal disease and renal aging.
Later in mouse models, they still saw success overall, but it was a bit more subdued.
Today, mouse models are still created within this school of thought.
While current protocols have shown the functionality of mature cells mostly in vitro, transfer of cells into the aforementioned humanized mouse models is still hampered by the rather low output of differentiated cells from PSC sources.
Moreover, our results show that, in a mouse model, treatment can still be effective with a 2- to 4-h interval between toxin and antitoxin injection.
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