Sentence examples for mouse midbrain sections from inspiring English sources

To investigate whether the ventral mesencephalic flexure expression of Rmst is indeed associated with dopaminergic neuronal lineage, we carried out further in situ hybridisation on mouse midbrain sections from E11.5 to E14.5, the developmental time window when the dopaminergic neuron territory is established (Fig. 3).

First, QC immunohistochemistry was performed on mouse coronal midbrain sections at the level of bregma −3.1 to −3.8 mm.

Serial transverse sections of mouse midbrain were cryosectioned (30 µm using a Leica CM1850) and every fourth serial section was immunostained with anti-Iba1 (1∶500, WAKO Chemicals) and visualized by using the chromogen diaminobenzidine.

Fig. 1B illustrates double immunolabeling for TH and Sim1 on fixed paraffin sections from mouse midbrain at the area of substantia nigra pars compacta (SNc) and ventral tegmental area (VTA).

We determined DCC antibody specificity by performing Western blot analysis on E17 whole brain tissues lysate from dcc −/− and +/+ mice and by immunohistochemistry on midbrain sections of E17 dcc −/− embryos.

Coronal midbrain sections (30 µm) were prepared from 4-5 month G2019S LRRK2 transgenic mice (TG; line 340) and non-transgenic littermate mice (NTG).

N-terminal cleavage of OPA1 is also observed in vivo in aged rat and mouse midbrain and hippocampal tissues.

DCC immunofluorescence on midbrain sections of E17 dcc −/− embryos revealed no immunoreactive signal (Fig. S1B), whereas high levels of DCC have previously been reported in wild type mice [11] [11].

The substantia nigra was dissected from 40 µm cryostat-cut midbrain sections.

Two midbrain sections per hemisphere were used for densitometric quantification of nigral α-synuclein immunoreactivity.

From human whole brain and mouse midbrain, several clones were detected by PCR.