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N-terminal cleavage of OPA1 is also observed in vivo in aged rat and mouse midbrain and hippocampal tissues.
Total RNA was also isolated from mouse midbrain and rat hippocampus.
Partial GABAB1k forms containing exon-intron junctions were also cloned from human cultured cell lines, mouse midbrain, and rat hippocampus.
After purifying total RNA from human cultured cell lines, mouse midbrain, and rat hippocampus, DNase treatment removed possible genomic DNA contamination.
We used the Pitx3-GFP allele in combination with fluorescence-activated cell sorting (FACS) to purify dopaminergic neurons from the developing mouse midbrain and compared the expression profile of the Pitx3-GFP+ and Pitx3-GFP− cell populations by hybridising labelled cRNA to Affymetrix mouse 430 array 2.0.
Although their cell-type specificity has not been demonstrated, expression of Tal1 and a related gene Tal2 have been detected in the mouse midbrain and r1 raising the possibility of unique or overlapping interactions with Gata2 (Elefanty et al., 1999; Mori et al., 1999; Herberth et al., 2005).
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HEPeak was also applied to real MeRIP-seq datasets from human HEK293T cell line and mouse midbrain cells and was shown to be able to recapitulate known mA distribution in transcripts and identify novel mA sites in long non-coding RNAs.
In turn, Fgf8 is important to maintain expression of Wnt1 in the early mouse midbrain [29] and Fgf8 beads induce expression of Wnt1 in chick embryos [30].
As shown in Figure 9, the most enriched motifs for the HEK293T cells and mouse midbrain cells are GGACH [ 10, 11], which were identified bound by methytransferase METTL3 and METTL14 [ 27].
From human whole brain and mouse midbrain, several clones were detected by PCR.
In the present study, we investigated the function of fMLP in neurodegeneration, using the well-established primary rat and mouse midbrain culture model of PD.
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