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We also examined 77 mouse microarray studies on the Affymetrix MOE430A platform, analyzing genes intersecting with the mouse Golden Path list (mm9) and the genes analyzed by Su et al. [60].
A small increase at the level of spermatogonia suggests that, in humans, MGAT1 is expressed in spermatogonia whereas GnT1IP/ MGAT4D is not, tallying with the data obtained from mouse microarray studies (Chalmel et al., 2007).
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Within these groups, 3 male-specific and 6 female-specific genes had a sex-bias of at least 2-fold at 8 wk and were confirmed as showing sex-specific expression in earlier mouse liver microarray studies (Table 4) [ 27, 32].
The circadian rhythmicity of many cartilage specific genes found in mouse is supported by microarray studies in rat cartilage under diurnal (LD cycle) conditions [ 72].
The few differences in gene expression between wild-type and Meig1-deficient mice detected by DNA microarray studies may reflect an artifact arising from low baseline levels of expression.
Microarray studies of mouse embryonic fibroblasts from wild-type and HSF1-null mice or a human cervical carcinoma cell line have shown that HSF1 controls only a subset of the genes altered by HS [ 7, 8] indicating that other inducible pathways play roles in regulating the Hsp genes.
A set of male C57BL/10 mice was used for the microarray studies, and a different set of female C57BL/6 mice was used for the qRT-PCR studies.
In microarray studies of mouse micromass cultures [ 8], 212 genes exhibited at least a ten-fold difference in gene expression as the cultures differentiated.
We collected 2,276 circadian oscillating genes from multiple microarray studies in mouse livers, and integrated their circadian peak and trough information [ 39].
Consistent with our finding in mouse liver, a microarray study of gene expression in postnatal rat liver (ages ranging from 2 wk to 104 wk) reported very few sex-specific genes at 2 wk and 5 wk.
For microarray studies, three mice per group at 15-16 mofths of age were sacrificed, and their livers were flash frozen.
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