Total RNA for the mouse (C57BL/6J) microarray experiments was isolated from NIH3T3 cells (fibroblast cell line), SL10 cells (fibroblast cell line), brain, heart, intestine, kidney, liver, lung, placenta (d.p.c. 10.5 and 13.5), spleen, stomach, testis, and thymus.
CRF designed and performed the mouse crosses and microarray experiments with the HG2D and HG11 congenic strains.
Consequently, 80 human, mouse and rat microarray experiments (datasets) from Gene Expression Omnibus (GEO) database met the selection criteria.
S.S., B.B., L.S., G.O., C.d.L., L.N.L., and D.B. performed the mouse functional and microarray experiments; N.S. and E.M. contributed to the bioinformatics analyses; and M.D., E.N., L.S., S.C., A.F., and E.B. contributed to the human vaccines studies.
First, we used the RNA samples of the 8 mice enrolled in microarray experiments (Derivation group).
Primary cortical neuron cultures were prepared from C57BL6/J (for qRT-PCR) or BALB/cJ@Rj (for microarray experiments) mouse embryos, as previously described (Samari et al., 2013).
Our recent work applied an automated high-throughput method to classify genes with bimodal expression profiles within the mouse genome based on microarray experiments performed on healthy tissues using the Affymetrix MGU74Av2 microarray platform [ 1].
Microarray experiments with mouse mammary gland and the same Affymetrix Genechip array MG-U74Av2 are available at public data bases [ 9, 36].
Microarray experiments with mouse strains that exhibited both high and low levels of response to inhaled lipopolysaccharide compounds (which trigger immune responses) identified about 500 genes that were responsive in at least one of the strains.
