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For mouse, microarray data forming a "tissue atlas" have been generated [12].
These mouse microarray data (and those for patients, see below) have been deposited in NCBI's Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) and are accessible through GEO Series accession number GSE6377.
In view of conserved cellular aspects of spermatogenesis, we anticipate that most genes will show conservation of their expression patterns at subsequent steps of spermatogenesis, between mouse and primates, and we used the available mouse microarray data to gain information about spermatogenic gene expression patterns in whole chimpanzee and human testes.
Mouse microarray data were obtained from the Genomics Institute of the Novartis Research Foundation SymAtlas.
We next extend our correlation analysis to human and mouse microarray data.
Our mouse microarray data have been deposited at NCBI/GEO, with the GEOSET number GSE54054.
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From these human-on-human, mouse-on-human, mouse-on-mouse and human-on-mouse microarray data, we generated lists of probes sets that had the potential to hybridise across species, as described in the Supplementary Methods.
The expression of the Slc2a4 gene in liver was significantly down-regulated in supplemented mice; microarray data showed consistent decreased Slc2a4 mRNA abundance due to adequate selenium and folate supplementation, with 8 separate probes for this gene on the array showing a >2 |fold-change| (range −2.10 to −2.39).
Mouse microarray expression data from [ 65] were obtained from the authors' website (http://hugheslab.ccbr.utoronto.ca/supplementary-data/Zhang/).
A similar trend analysis shown in Figure 5A is also performed by comparing human and mouse microarray expression data in matched tissues.
This article describes an evaluation and reanalysis, by two independent laboratories, of previously published TGx mouse liver microarray data for a triazole fungicide, propiconazole (PPZ), and the anticonvulsant drug phenobarbital (PB).
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