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Mouse miRNAs display a similar distribution.
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CIS-associated mouse miRNA precursors have 96% identity with human sequences whereas non-CIS-associated miRNAs display 91% identity, slightly lower than the average identity level for all mouse miRNA precursors (92%).
Furthermore, these inducible miRNAs display different specificity under different stresses.
Using this approach, 85% miRNAs displayed ΔΔCt < 1.2.
Thirty-two miRNAs displayed internal nucleotide changes with a frequency >5%.
The remaining 21 novel miRNAs displayed no obvious sequence homology with known miRNAs.
Figure 2: Many secreted nematode miRNAs have identical seed sites to mouse miRNAs.
Given that many of the nematode miRNAs are homologues to mouse miRNAs, it is tempting to speculate that these could tap into existing miRNA regulatory networks in host cells.
The C.elegans miR-67 mimic was selected as suitable negative control due to its minimal sequence identity with mouse miRNAs and the confirmed absence of detectable effects on examined mouse miRNA functions (www.http://dharmacon.gelifesciences.com/).
In support of this, we show with a reporter assay that three of the secreted nematode miRNAs that have identical seed sites to mouse miRNAs can together downregulate DUSP1 through conserved sites in its 3′UTR.
Thus far, >2500 human miRNAs (hsa-mir) and 1900 mouse miRNAs (mmu-mir) have been identified and described at the miRBase website (http://www.mirbase.org, released in Nov. 2013).
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