A μParaflo microfluidic chip, which included 617 unique, mature, mouse miRNA, based on the Sanger miRBase Release 12.0, was used for assay.
Each microarray contains sixteen to twenty oligonucleotide probes for each of the 627 mouse miRNAs and 39 mouse viral miRNAs based on the Sanger database version 12.
MatureBayes [ 23] uses a Naive Bayes classifier to identify the location of human and mouse mature miRNAs based on information specific to individual nucleotide.
Briefly, epoxy-coated Nexterion glass slides were spotted with eight replicates of a locked nucleic acid (LNA) probe set consisting of 2,367 probes against human, mouse, and rat miRNAs based on miRBase 16.
TLDA allows for quantitation of 335 mouse and 226 rat miRNAs based on Sanger mirBase v. 10 annotations.
PicTar has target prediction information for human miRNAs based on conservation in mammals (human, chimpanzee, mouse, rat, dog) [30].
We evaluated the sequence conservation of the miRNAs based on the whole genome alignments of human, rhesus, mouse and 14 other vertebrate species by calculating the per-site conservation probability of the human pre-miRNAs.
Microarray analysis of mouse cells incubated with nematode exosomes in vitro identifies Il33r and Dusp1 as suppressed genes, and Dusp1 can be repressed by nematode miRNAs based on a reporter assay.
The annotation of miRNAs based on the appearance of miRNA* would often miss many true miRNAs.
Our analysis, using both NG2-KO mice and a miRNA based silencing approach, clearly demonstrates that NG2 is not required for PDGF-B to efficiently induce and maintain gliomas from neural progenitors.
