Sentence examples for mouse miRNA sequences from inspiring English sources
Known mature human and mouse miRNA sequences were obtained from the miRBase database (http://microrna.sanger.ac.uk/cgi-bin/sequences/browse.pl).pl
Recently, the Sanger Institute released the latest version of their database of known miRNAs (miRBase 14.0; Sep 2009, http://microrna.sanger.ac.uk); 786 mature mouse miRNA sequences are currently reported.
313 mature human and 233 mature mouse miRNA sequences were obtained from miRBase release 7.0 [35] (www.sanger.ac.uk/software/Rfam) and are available as Supplementary Table S8.
The recently released (June 2014) miRBase database version 21 hosts 1193 mouse miRNA sequences.
We investigated the correspondences of the predicted 7-bp motifs with mature mouse miRNA sequences documented in the miRNA registry.
Conserved mouse miRNA sequences within the microRNA Registry were used to conduct BLAST searches of the existing zebrafish and fugu genomes.
To validate this hypothesis we used non-redundant set of known human, dog and mouse miRNAs sequences listed in miRBase 13.0 to search for highly similar DNA sequences in the bovine genome.
Specifically, sequence alignment was performed using Burrows-Wheeler Aligner (BWA) [ 41] against mouse mature miRNA sequences (downloaded from miRBase (Release 20: June 2013; http://www.mirbase.org/), while allowing one mismatch between read and reference.
In order to extend annotations, human, bovine, dog, pig and mouse miRNAs precursor sequences were aligned to the horse genome by BLAST (version2.2.25).
In miRGate, we stored human, human viruses, mouse and rat miRNA sequences (Table 2), as well as other available information such as cleavage data from pre-miRNAs to mature miRNAs, genomic coordinates and family names.
The sequences of mmu-miR-199a-5p mmu-miR-199a-5p mmu-miR-199a-5p mmu-miR-199a-5pmmu-miR-199b in mouse miRNA corresponded to the sequences of hsa-mmu-miR-199ba-mmu-miR-200asa-mmu-miR-200ba-min-200a, and hsa-mouse00b in humiRNAiRNA, respecorrespondedle S3).
