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The human and mouse membrome datasets are available through the Membrome homepage (http://www.membrome.org/) and correspond to subsets of the SymAtlas content restricted to known membrane trafficking components.
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Here, we describe the use of the SymAtlas web‐application and the Membrome datasets to help explore trafficking GTPase function.
Considering the fragmentary nature of the current reductionist approaches in elucidating trafficking component functions, the membrome datasets provide a more focused systems biology perspective that not only complements our current understanding of transport in complex tissues but also provides an integrated perspective of Rab activity in controlling membrane architecture.
Based on our results, we then compared pig and mouse in vivo datasets with human PED datasets.
Finally we obtained 14036 and 13134 human and mouse gene datasets with AP annotations, respectively.
Table 2 describes the datasets used, which include 12 human cancer datasets, five mouse cancer datasets, one rat cancer dataset, and one dataset from zebrafish.
The Yeast dataset includes 44 functional association networks, the Human dataset includes 8 networks, the Mouse dataset consists of 10 networks, and the Fly dataset has 38 networks.
The mouse datasets is extracted from a set of combined SNPs from the 10 K GNF (http://www.gnf.org/) mouse dataset and the 140 K Broad/MIT mouse dataset (Wade and Daly, 2005).
This resulted in 13,032 and 9,062 unique genes for the human and mouse datasets, respectively.
The human and mouse datasets were normalized using the Robust Multichip Average (RMA) algorithm.
Note p-values in Figure 4 are not directly comparable between Drosophila and mouse datasets.
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